HyperShear™ Static Mixers

• Static Mixers are available in Standard HPLC, Ultra HPLC, Biocompatible PEEK and In-Line Combo Series.
• Standard HPLC Mixers are constructed from 316 stainless steel with volumes ranging from 0.5 μL to 15.0 mL and with pressure up to 6,000 PSI.
• Ultra HPLC Mixers are constructed from 316 stainless steel with volumes ranging from 0.5 µL to 1.5 mL and pressure up to 18,000 PSI.
• Biocompatible Mixers are constructed from PEEK as wetted material with volumes ranging from 0.5 μL to 1.5 mL and with pressure to 3,000 PSI.
• Temperature rating is 40°C for all volumes; 0.5 μL to 15.0 mL, and for all Standard HPLC, Ultra HPLC, Biocompatible PEEK and all In-Line Combo Series.
• High Temperature rating above 40°C with Max 80°C is available for 0.5 μL to 1.5 mL for Standard HPLC (6,000 PSI). High Temperature & Ultra High Pressure (12,000 PSI) is available for Micro Series (0.5 μL - 25 μL). 
• In-Line Combo Mixers are suitable for seeking to optimize mixing volumes at flow rates below 100 μL/min. and have the ability to customize a mixing volume which is perfectly tuned to match the separation system.
• ASI Hypershear Mixer enables pharmaceutical assay.  read this article
• Static Mixer Development Kits for the equipment manufacturer or the scientist that needs to optimize a separation or process, are available.

Static Mixer Application Notes and Volume Selection Guide in PDF and Static Mixer Selection Chart below on this page

ASI vs Competitor Comparison

Static Mixer Performance Data 1 - 8

Static Mixer Performance Data 9 - 10  Shimadzu LC-10ADVP pump data

Static Mixer Performance Data 11  ASI Hypershear Mixer enables pharmaceutical assay.

Chromatography Forum, HPLC Static Mixers  Discussions about HPLC, CE, TLC, SFC, and other "liquid phase" separation techniques.

Journal of Chromatography, ASI 250 μL Static Mixer  The application of ASI 250 µL mixer to neutralize an antibody – protein system 

Linear Gradients - High Pressure Mixing

The best choice is usually a compromise between delay volume and mixing noise - more mixing volume will reduce mixing noise but at the cost of more delay volume.  The upper limits to mixing volume will be the maximum delay time that can be tolerated, and possible distortion (tailing) of the gradient at the beginning and end of the gradient. The lower limit will be defined by the amount of mixing noise that can be tolerated. The following cartridge volumes are a compromise between these two limits.

For illustration ONLY

1. When mixing noise is limiting, use Flow Rate, Column 1

2. Mixing delay volume is sometimes referred to as dead volume. It affects peak shape or dispersion. Use Flow Rate, Column 2 when this is critical.