| Question 1 | “What mixer will fit my app? I want to optimize it to get as much mixing as I can without creating too much noise or dead volume. How do I know what will be the optimal mixer volume?” |
| Answer 1 | There is no one correct answer for this question. Our Static Mixer Selection Chart will provide a good estimate. However, to refine the solution the user will need to keep in mind that more mixing volume will create more delay time and less mixing noise. To optimize the mixer volume, the scientist must tune the mixer volume to meet performance specifications of the system method.To save time and development effort, you might consider the new ASI Static Mixer Development Kit. |
| Question 2 | “How do I select the best fit mixer volume for my application? |
| Answer 2 | HPLC– select smallest volume to limit dwell time & peak dispersion Non-reactive reagent mixing– select by largest flow rate Reactive mixing– select larger volume – up to 10x flow of rate-limiting reagent. Other– contact ASI! DWELL volume(supply app note for measuring dwell volume) If dwell volume of system is known, then pick a mixer with that volume. If dwell volume is not known, then determine it (see app note) and match the experimentally determined volume. There is no one correct answer for this question. Our Static Mixer Selection Chart will provide a good estimate. However, to refine the solution the user will need to keep in mind that more mixing volume will create more delay time and less mixing noise. |
| Question 3 | I would like to incorporate 50 µL mixer in a HPLC system to mix two solutions with a 6:1 concentration ratio (0.2 M permanganate at pH 14 + 0.03 M sodium iodide, both aqueous solutions), working between 0.5 mL/min. and 2 mL/min. flow rate. Could you please inform me about the approximate degree of mixing achieved in this case? |
| Answer 3 | PEEK and chemical compatibility are easy — we have PEEK mixers and they are compatible with your reagents. More mixing volume will create more delay time and less mixing noise. Not enough mixing may result in a failed experiment. We can’t accurately predict if 50 µL is enough for your application. Based on flow only, you might consider a 350µL mixer. However, you must confirm if this general approach will meet your performance specifications. |
| Question 4 | I was wondering if you could tell me if ASI Dynamic Mixers are compatible with Agilent 1100/1200/1260 HPLC. |
| Answer 4 | Our Dynamic mixers were developed for use with the Agilent 1100 series pumps, especially when using TFA or other difficult mixing applications. |
| Question 5 | We own a ternary mixer from you and have observed the effect, that the order in which our eluents are connected has an influence on reproducibility. Can you tell us something about the geometry of the "head" of the mixer to be able to understand this effect? |
| Answer 5 |
There is a small conical shaped volume at the inlet of the mixer that acts as a collection point for incoming solvents – prior to blending in the mixer cartridge. Due to inevitable pressure pulsations in each of the 3 streams, there can be a fluctuation in the composition in this collection node. This effect is more pronounced when one of the flow streams is much smaller than the others. Either reduce pressure pulsations, or try the addition of a small volume prior to the mixer, for instance a few inches of .030” id tube. |
1. When mixing noise is limiting, use Flow Rate, Column 1
2. Mixing delay volume is sometimes referred to as dead volume. It affects peak shape or dispersion. Use Flow Rate, Column 2 when this is critical.
| Flow Rate | Maximum Operating Pressure | ||||
|---|---|---|---|---|---|
| Column 1 | Column 2 | Product Links | |||
| The lower limit will be defined by the amount of mixing noise that can be tolerated | The upper limit will be the maximum delay time that can be tolerated | ASI Mixer Cartridge Volume | SST 6K PSI | SST 18K PSI | Biocompatible 3K PSI |
| < 1µL/min. | 0.5 µL | 40X-000.5 | 40X-000.5HP | 40X-000.5B | |
| 0.5 µL/min. | 2 µL/min. | 1 µL | 40X-0001 | 40X-0001HP | 40X-0001B |
| 1 µL/min. | 5 µL/min. | 2 µL | 40X-0002 | 40X-0002HP | 40X-0002B |
| 2 µL/min. | 10 µL/min. | 5 µL | 40X-0005 | 40X-0005HP | 40X-0005B |
| 5 µL/min. | 20 µL/min. | 10 µL | 40X-0010 | 40X-0010HP | 40X-0010B |
| 10 µL/min. | 50 µL/min. | 25 µL | 40X-0025 | 40X-0025HP | 40X-0025B |
| 20 µL/min. | 100 µL/min. | 50 µL | 41X-0050 | 41X-0050HP | 41X-0050B |
| 40 µL/min. | 160 µL/min. | 80 µL | 41X-0080 | 41X-0080HP | 41X-0080B |
| 60 µL/min. | 200 µL/min. | 100 µL | 41X-0100 | 41X-0100HP | 41X-0100B |
| 100 µL/min. | 250 µL/min. | 150 µL | 41X-0150 | 41X-0150HP | 41X-0150B |
| 150 µL/min. | 400 µL/min. | 200 µL | 41X-0200 | 41X-0200HP | 41X-0200B |
| 200 µL/min. | 500 µL/min. | 250 µL | 41X-0250 | 41X-0250HP | 41X-0250B |
| 500 µL/min. | 2 mL/min. | 350 µL | 42X-0350 | 42X-0350HP | 42X-0350B |
| 1 mL/min. | 5 mL/min. | 500 µL | 42X-0500 | 42X-0500HP | 42X-0500B |
| 2 mL/min. | 10 mL/min. | 800 µL | 43X-0800 | 43X-0800HP | 43X-0800B |
| 5 mL/min. | 20 mL/min. | 1.0 mL | 43X-1000 | 43X-1000HP | 43X-1000B |
| 10 mL/min. | 30 mL/min. | 1.5 mL | 43X-1500 | 43X-1500HP | 43X-1500B |
| 20 mL/min. | 50 mL/min. | 3.0 mL | 45X-30X0 | ||
| 30 mL/min. | 80 mL/min. | 6.0 mL | 45X-60X0 |
||
| 50 mL/min. | 100 mL/min. | 9.0 mL | 45X-90X0 | ||
| 80 mL/min. | 1 L/min. | 15 mL | 45X-150X0 | ||